Filtering of fermented functional sugars 

Objective: To remove solid impurities such as cells, cell debris, and insoluble substances from the fermentation broth through filtration, in order to obtain a more pure fermentation broth. 

Method: 

Use appropriate filtering media, such as silica sand and cellulose as auxiliary filters, to make the filter cake loose and increase the filtration speed. 

By adjusting the pH value, the charge properties of the easily adsorbed molecules can be changed, thereby reducing blockage and pollution. 

Heat treatment can reduce the viscosity of the liquid, cause the protein to coagulate, decrease the viscosity of the fermentation broth, and facilitate filtration. 

Add flocculants, such as polymer macromolecules, which exert electrostatic attraction and other forces to cause the colloidal particles to form a flocculated state, thereby reducing the viscosity of the fermentation solution. 

Extraction of fermentable sugars 

Objective: To extract fermentation functional sugars from the filtered fermentation broth. 

Method (taking the fermentation product of Ganoderma lucidum as an example): 

For extracellular polysaccharides (polysaccharides in the fermentation broth), they can be precipitated by adding ethanol, and then filtered to obtain crude polysaccharides. 

For intracellular polysaccharides (mycelial polysaccharides), the mycelium can be dried and ground finely, and then the polysaccharides can be extracted using distilled water. 

The acid extraction and alkaline extraction methods can also be used to extract polysaccharides from insoluble substances in the fruiting bodies, mycelium, or fermentation broth. 

Purification of fermented functional sugars 

Objective: To further remove impurities from the extract and improve the purity and quality of the fermentation functional sugars. 

Method: 

The protein in the extraction solution was removed by using the Sevag method for protein denaturation. 

Centrifuge to obtain the supernatant. After the precipitated part is dried, relatively pure fermentation functional sugars are obtained. 

Gel chromatography (HPLC) can be used to determine the content of polysaccharides. Quantification is carried out using the external standard method to ensure the purification effect. 

Notes for Attention

Throughout the entire process, it is necessary to pay attention to the hygiene and sterility of the operating environment to prevent microbial contamination. 

Strictly control parameters such as pH value, temperature, and stirring speed to ensure the effectiveness of filtration, extraction and purification. 

Depending on the specific types of fermentable sugars and the requirements of the particular process, adjustments and optimizations may be necessary for the above steps and methods. 

In conclusion, the filtration, extraction and purification of fermentative functional sugars is a complex and meticulous process that requires comprehensive consideration of multiple factors and technical means to ensure the quality and purity of the final product. 

For many years, Wuhan Bona has been dedicated to the application process development of membrane separation technology. By integrating processes, membranes and equipment, it has accumulated rich experience in product purification and engineering-scale expansion. It not only solves manufacturing challenges but also improves product quality, and together with customers, it enhances the technical level of the industry.